In-depth GlycoProfiling, PTM analytics and protein analytics

N- and O-Glycoprofiling Service

  • Innovative combination and automation of analytical methods for high-throughput analysis of complex glycosylation patterns
  • Precise glycan structure identification for isobaric structures by use of CID-MSMS
  • Precise quantification based on sensitive fluorescence detection
  • Calculation of biologically relevant glycan parameters such as fucosylation, sialylation etc.
  • Comparison of results to reference standards
  • Analysis of complex glycan patterns of >100 individual structures
Workflow automated glycoprofiling

Site-specific glycoprofiling
  • Innovative combination of PeptideProfiling and GlycoProfiling for precise site specific identification and quantification
  • Purification and separation of glycopeptides
  • Fractionation of glycopeptides comprising the same glycosylation site
  • Profiling of glycans released from one glycosylation site
  • Precise glycan structure identification by use of CID-MSMS
  • Precise quantification based on sensitive fluorescence detection
Accessories for glycoprofiling
  • Glycoprotein standards for glycoprofiling
  • GlycoLib – the glycan library
  • MSMS spectra library comprising approx. 300 mAb glycans and 750 glycans from other proteins
  • Characteristic fragments for identification of glycoepitopes
  • Intensity patterns of glycan fragments for identification of glycan structures

PTM Analytics

Identification and quantification of PTM’s by high-resolution UPLC-ESI-QTOF mass spectrometry:

Integrity of the protein by peptide analysis
  • Peptide mapping
  • Sequence verification
  • Truncation
  • Disulfide bridges
Overall and site-specific analysis of PTM‘s
  • Oxidation
  • Deamidation
  • Lysine clipping
  • Pyroglutamate
  • Disulfide bridges
Analysis of special PTM’s
  • Amidation
  • Glycation
  • Sulfation
  • Phosphorylation
  • Isomerisation
  • N-Terminal acetylation
  • Acylation
  • Hydroxylation
  • Racemisation
  • etc.
Intact protein and middle-up analysis
  • Molecular mass
  • Integrity of the amino acid sequence and glycosylation
  • Fragments
  • Truncation
Occupancy of glycosylation sites

Quantification of the amount of glycans attached to a glycosylation site or to the complete protein:

Protein Analysis

 

Molecular mass – analysis of the intact antibody
  • With or without glycosylation
  • Truncation analysis
  • Middle-up analysis
    • fragments
    • integrity of the amino acid sequence
    • glycoprofiling

PK Analysis

 

MS based PK analysis
  • Identification and quantification of proteins in serum
    • peptidase digestion
    • MS profiling of characteristic peptides
    • structure verification by MS/MS experiments
    • absolute quantification based on MS peak areas
  • Analysis according to GCLP guidelines

GlycoLib – the Glycan Library

Fast and unambigious assignment of both simple and complex glycan Patterns:

GlycoLib and standards are available for DataAnalysis (Bruker)

We offer ISO 9001 compliant services to ensure controlled quality of PTM Analytics.

Dr. Sven Bahrke
t: +49 (0)30 9489 2600
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