WE CARE ABOUT YOUR GLYCOPROTEIN Most pharmacologically relevant proteins (such as growth factors, cytokines, hormones and antibodies) are glycosylated. More and more, the correct human glycosylation and optimal sialylation of a recombinant protein is considered to be essential for full bioactivity, half-life, stability or immunogenicity of that glycoprotein. GLYCOTOPE offers a broad range of customized services in state-of-the art glycoanalytical and glycoengineering techniques. If you want to generate your pharmaceutical protein or a new improved generation of a generic blockbuster with a fully human glycosylation, in a higher active form, with an optimized serum half-life or reduced dosing, we offer new effective expression and engineering technologies to achieve these goals. GlycoExpress is our human cell line-based platform that allows protein expression with fully human glycosylation and optimized sialylation. For the generation of new expression cell lines for defined glycosylations and the analysis of the structure and function of your glycoprotein make use of the extensive know-how and experience from us and our partners in GlycoEngineering, GlycoAnalytics, GlycoProfiling, Pharmacokinetics and Bioassays. GlycoAnalytics and GlycoProfiling You need to know the glycosylation of your protein or your cellular expression system? We offer together with our academic partner (Prof. Dr. Reutter, Charitè, Campus Benjamin-Franklin, Berlin):

	Structural analysis of carbohydrate chains using HPLC- and MS-techniques
	Analysis of the cellular glycosylation machinery using RT-PCR,
	our GlycoProfiler, and enzymatic assays
	Profiling by using lectins and carbohydrate-specific antibodies
	Analysis of the sialylation degree
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GlycoEngineering Do you want to generate a new more effective generation of a generic or new glycoprotein with fully human glycosylation and a new patent protection ?

	Use our GlycoExpress Platform to express high amounts of your protein with fully human glycosylation.
	Use our GlycoExpress Platform to select the optimal degree of sialylation
	You will receive a glycoprotein with higher biological activity, longer physiological half-life and less side-effects.
You are restricted to your own cellular expression system, but you need to optimize glycosylation of your recombinant protein?
	We are glycoengineering your cell line to produce the desired glycosylation.
	We also modify and refine your protein in vitro (e.g. PEGylation).
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Evaluation of Glycoprotein Properties, Activities & Pharmacokinetics We offer a broad range of techniques, e.g.:

	Cellular assays, e.g. cell adhesion, proliferation, migration, differentiation & cell death
	Determination of serum half-life and biodistribution in mice & rats
	Determination of immunogenicity in vitro and in vivo
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Company Contact GLYCOTOPE GmbH Robert-Rössle-Str. 10 13125 Berlin Germany Hans Baumeister, Ph.D. Chief Operating Officer

Telephone:   Fax: E-mail:

+49-30-941084-207 +49-30-9489-2612 hans.baumeister@glycotope.com