Glyco Technology

GlycoEngineering:

Existing production cell lines can be glycoengineered to optimise the glycosylation pattern in order to achieve the desired glycosylations either to obtain the activity, serum half-life and/or stability of your protein (e.g. growth factor or antibody), or to avoid immunogenicity.
We offer our extensive know-how and service to achieve these goals.

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GlycoAnalytics and GlycoProfiling:

GlycoAnalytics and GlycoProfiling comprises the whole range of analytical technologies for the characterization of glycoproteins and the glycosylation machinery of cell lines:

  		•  the structural analysis of carbohydrate chains and their sialylation degree and type
    using lectins and highly specific antibodies, a series of chromatographic techniques
    (e.g. anion exchange HPLC, hydrophilic HPLC, RP-HPLC, size exclusion
    chromatography, HPAEC-HPLC, multistep HPLC, TLC), and mass spectrometry
    MALDI-MS, GC-MS, ESI-Q-TOF-MS/MS) in combination with chemical and
  		• the analysis of the glycosylation machinery (e.g. transferases, glycosidases,
    sugar transporter, epimerases) of cells and tissues using RT-PCR, DNA-Array
    Technology and lectin and antibody staining in combination with chemical and
    enzymatic treatments.

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    Bioassays:

    The function of the glycoproteins is tested in molecular or cellular bioassays. GLYCOTOPE has profound experience in these assays amongst them e.g. cell adhesion, proliferation, migration, differentiation and activation, and cell death assays. We offer the broad range of existing tests or use our know-how for the development or adaptation and optimisation of new tests.

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    Immunogenicity Tests:

    We analyse the immunogenicity of the glycoprotein on the humoral and cellular immune system. We use in vitro cellular immune assays, e.g. dendritic cell based assays, T cell assays, ADCC, CDC, macrophage and NK cell assays, and together with our partner EPO-GmbH various mouse models, e.g. immune competent mice, nude mice and NOD-SCID mice together with reconstitution of a human immune system using PBMC, testing their cellular immune response and humoral immune response (antibody titres, isotypes and specificities).

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    Pharmacokinetics:

    We test the serum-half life and biodistribution in mice and rats and optimise it in combination with above technologies.

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