GlycoProfiling, PTM analytics, protein analytics, Pk under GCLP based on LC-MS for characterization of proteins, quality control for development, product release assays, clinical Services

GlycoProfiling

  • Innovative combination and automation of analytical methods for high-throughput analysis of complex glycosylation patterns
  • Precise glycan structure identification for isobaric structures by use of CID-MSMS
  • Precise quantification based on sensitive fluorescence detection
  • Calculation of biologically relevant glycan parameters such as fucosylation, sialylation etc.
  • Comparison of results to reference standards
  • Analysis of complex glycan patterns of >100 individual structures
  • Highly experienced with GxP environment

 

Site-Specific GlycoProfiling

  • Innovative combination of PeptideProfiling and GlycoProfiling for precise site specific identification and quantification
  • Purification and separation of glycopeptides
  • Fractionation of glycopeptides comprising the same glycosylation site
  • Profiling of glycans released from one glycosylation site
  • Precise glycan structure identification by use of CID-MSMS
  • Precise quantification based on sensitive fluorescence detection

 

 

GlycoLib – the Glycan Library

fast and unambigious assignment of both simple and complex glycan Patterns

PTM Analytics

  • Methods based on UPLC coupled to ESI-QTOF-MS/MS
  • High resolution chromatography and mass spectrometry for reliable identification and quantification of PTM’s
  • Peptide mapping / Sequence verification
  • Site-specific analysis of:

Oxidation
Deamidation
Lysine clipping
Pyroglutamate
Disulfide bridges

  • Overall and site-specific analysis of special PTM‘s such as:

Amidation
Glycation
Sulfation
Phosphorylation
Isomerisation
N-Terminal acetylation
Acylation
Hydroxylation
Racemisation

Protein Analysis

Molecular mass – analysis of the intact antibody

  • With or without glycosylation
  • Truncation analysis
  • Middle-up analysis

fragments
integrity of the amino acid sequence
glycoprofiling

 

Pk Analysis

MS based Pk analysis

  • Identification and quantification of proteins in serum

peptidase digestion
MS profiling of characteristic peptides
structure verification by MS/MS experiments
absolute quantification based on MS peak areas

  • Analysis under GCLP guidelines